Quanta Biosciences Perfecta Sybr Fastmx Lrx 1250 95074-012 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Quanta Biosciences Perfecta Sybr Fastmx Lrx 1250 95074-012 / 101414-286 is part of a wide selection of Quanta Biosciences Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction. Combining excellent quality with an affordable price, the Quanta Biosciences Perfecta Sybr Fastmx Lrx 1250 95074-012 / 101414-286 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction please do not hesitate to contact our customer service for further guidance.
This unique combination of proprietary buffer, stabilizers, and AccuFast* Taq DNA polymerase delivers maximum PCR† efficiency, sensitivity, and specificity, along with a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification.
AccuFast Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a unique mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (>48 hours at room temperature). Similar to our AccuStart Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200bp is complete in less than 20 seconds at 60°C.
Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX* reference dye. Platforms not requiring passive reference dye include Roche LightCycler* 480, Bio-Rad* iQ*, MyiQ*, iQ5, Opticon*, and Chromo4*, Corbett Rotor-Gene*, Eppendorf* Mastercycler*, and Cepheid* SmartCycler*. Kits with Low ROX reference dye are for use with platforms including AB 7500 and Stratagene* Mx*. Kits with ROX reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne*.