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The modified stain kits employ a modification of the Kleihauer technique for fetal hemoglobin. The new technique employs a procedure based on the resistance of fetal hemoglobin to acid conditions. This test does not require heat and can be performed at room temperature, while the original technique requires mixing and heat. Fixation of the smear with ethanol allows hemoglobin to precipitate within the red blood cells (RBCs). Air-dried and fixed blood films are then treated with a citric acid/phosphate buffer of low pH to rapidly elute adult hemoglobin. The RBCs containing fetal hemoglobin (HbF) remain precipitated and stain bright red with eosin, resulting in easy differentiation. RBCs without HbF appear as colorless "ghosts". The hematoxylin stains the nuclei of white blood cells (WBCs) blue to prevent misinterpretation as eosin-positive cells.
These solutions are made from certified dyes (when applicable). For in vitro diagnostic use only.
Ordering Information: Stain kit volume represents the volume for each component in the kit.
| Description: | Solution II - Disodium Phosphate, 0.2 M |
| Volume, mL: | 250 |
| Eng No.: | 5706 |
| Unit: | Pack of 2 |