
Code: Y2-9LJ-27-5325-01
MPN: 27-5325-01
GE Microspin G-25 Columns PK50 27-5325-01 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. GE Microspin G-25 Columns PK50 27-5325-01 / 95017-621 is part of a wide selection of GE Protein analyzer reagents. Combining excellent quality with an affordable price, the GE Microspin G-25 Columns PK50 27-5325-01 / 95017-621 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Protein analyzer reagents please do not hesitate to contact our customer service for further guidance.![]()
The MicroSpin G-25 columns are designed for the rapid buffer exchange/desalting of PCR†products and other DNAs in a volume of 10-100µL using spin column chromatography. They are also excellent for rapid purification of newly synthesized oligonucleotide >=10-mers in 100-150µL of deprotection solution. Supplied ready for use, columns require less than four minutes from sample application to collection of purified product. Prepacked with Sephadex* G-25 DNA grade medium and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide. They can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides in a volume of 10-100µL.
The MicroSpin G-50 columns are designed for the rapid purification of labeled DNA (>=20 bases) from unincorporated labeled nucleotides in a volume of 25-50µL using spin column chromatography. Columns are prepacked with Sephadex* G-50 grade medium pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide. Columns can also be use for buffer exchange/desalting of enzymatic reactions (10-100µL) following heat denaturation and phenol extraction.
MicroSpin S-200 HR columns are designed for the rapid purification of labeled single-stranded or double-stranded DNA fragments >=50 bases in length in a volume of 51-100µL using spin column chromatography.
MicroSpin S-300 HR columns are designed for the rapid purification of PCR products (>=100bp) from unincorporated primers (<=20-mers) and nucleotides using spin column chromatography. Columns are prepacked with Sephacryl* S-300 HR medium pre-equilibrated in TE buffer. The S-300 HR columns accommodate 25-50µL for post-PCR cleanup prior to sequencing, and are useful for purification of alkaline-denatured plasmid DNA prior to sequencing. Columns require less than four minutes from sample application to the collection of purified product.
MicroSpin S-400 HR columns are designed for the rapid purification of PCR products (>=200bp) from unincorporated primers (<=32-mers) and nucleotides using spin column chromatography. Columns are prepacked with Sephacryl S-400 HR medium pre-equilibrated in TE buffer. The S-400 HR columns accommodate 25-50µL for post-PCR cleanup prior to cloning or a second amplification reaction, or 51-100µL for all other applications. Columns require less than four minutes from sample application to the collection of purified product.
Note: MicroSpin G-50 columns are not intended for use with ultrasalt-sensitive automated DNA sequencers.
| Description: | MicroSpin G-25 Columns |
| GE No.: | 27-5325-01 |
| Unit: | Pack of 50 |