Product Info for GE Healthcare Life Sciences, Thrombin Protease, 27-0846-01, One unit of enzyme cleaves 90% of 100 A'ASg of a test GST fusion protein when incubated in 1 A~ PBS at 22Aa¹C for 16 h.

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Use for site-specific separation of the GST affinity tag from proteins expressed with pGEX-T vectors. The enzyme is purified from bovine plasma and is functionally free of plasminogen, plasmin, and other clotting factors. It can be used either following affinity purification or while proteins are bound to glutathione separation media.

Unit Definition: One unit of enzyme cleaves >=90% of 100µg of a test GST fusion protein when incubated in PBS at 22° (72°F) for 16 hours.

Specifications for GE Healthcare, Life Sciences, Thrombin, 27-0846-01:

Complete Packsize:	500 units
Application:	Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Unit Definition:	One unit of enzyme cleaves 90% of 100 A'ASg of a test GST fusion protein when incubated in 1 A~ PBS at 22Aa¹C for 16 h.
Vector pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-2TK:
Molecular Weight:	Mr 37000
Inhibitor:	Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, A'A¿1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF
Elution Buffer:	50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Cleavage Buffer:	PBS
Supplied As:	Lyophilized powder

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GE Healthcare Life Sciences, Thrombin Protease, 27-0846-01, One unit of enzyme cleaves 90% of 100 A'ASg of a test GST fusion protein when incubated in 1 A~ PBS at 22Aa¹C for 16 h. w/ Free S&H

Product Info for GE Healthcare Life Sciences, Thrombin Protease, 27-0846-01, One unit of enzyme cleaves 90% of 100 A'ASg of a test GST fusion protein when incubated in 1 A~ PBS at 22Aa¹C for 16 h.

Specifications for GE Healthcare, Life Sciences, Thrombin, 27-0846-01: