Lucigen Pezseq Hcamp Blunt Cloning Kit 40488-2

Lucigen Pezseq Hcamp Blunt Cloning Kit 40488-2 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Lucigen Pezseq Hcamp Blunt Cloning Kit 40488-2 / 95024-080 is part of a wide selection of Lucigen Bacteria competent cells. Combining excellent quality with an affordable price, the Lucigen Pezseq Hcamp Blunt Cloning Kit 40488-2 / 95024-080 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Bacteria competent cells please do not hesitate to contact our customer service for further guidance.

Designed for PCR† and cDNA cloning, as well as shotgun sequencing. The pEZSeq kits are optimized for the highest efficiency of blunt end cloning and sequencing using a blue/white vector. Each pEZSeq vector yields 99% recombinant clones with blunt, dephosphorylated ends, compared to common pUC based vectors that are only 40-70% efficient. The low background of blue colonies from pEZSeq vectors virtually eliminates robotic picking errors and greatly simplifies manual clone selection. The low background also allows transformants to be plated without blue/white screening in cDNA and shotgun library construction.

pEZSeq vectors are designed with strong transcriptional terminators on either side of the lacZ gene to reduce bias against toxic inserts. They also feature a minimal length of DNA between the cloning site and the primer binding sites, enabling the resulting sequence to contain 5-10% more insert sequence. The additional data can reduce the number of clones needed to assemble a contig and can add millions of extra bases to large-scale projects. In addition, the micro vector design minimizes unwanted transposition events, facilitates the cloning of larger inserts, and simplifies subsequent manipulations.

The lac promoter of pEZSeq vectors are constitutively active in E. cloni® 10G cells, so IPTG is not required for the blue/white screen. The lacq allele on the F' of 10GF' efficiently represses the lac promoter, allowing the user the option of adding IPTG to use the blue/white screen to assess cloning efficiency or omitting IPTG induction to reduce any cloning bias that may be created by high expression of the inserts.

Note: Kits with electrocompetent cells are recommended for use with Eppendorf*, Bio-Rad*, and BTX* electroporation cuvettes. They are not recommended for use with Invitrogen* cuvettes.

Specifications for Lucigen Pezseq Hcamp Blunt Cloning Kit 40488-2:

(Please note that the description and stock photo may not correspond to the specific model listed above)

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