Lucigen Ptrueblue Vector 42230-1

Lucigen Ptrueblue Vector 42230-1 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Lucigen Ptrueblue Vector 42230-1 / 95024-058 is part of a wide selection of Lucigen Polymerase chain reaction PCR cloning vectors or kits. Combining excellent quality with an affordable price, the Lucigen Ptrueblue Vector 42230-1 / 95024-058 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Polymerase chain reaction PCR cloning vectors or kits please do not hesitate to contact our customer service for further guidance.

The pJW168 vector is designed for in vivo insertion/removal of a DNA sequence through cre-lox site-specific recombination. The vector contains the cre-recombinase gene under the control of the IPTG-inducible lacUV5 promoter, as well as unique restriction sites for cloning and the gene for ampicillin selection. This vector allows DNA fragments to be integrated and then completely removed from the bacterial chromosome. Following recombination, growing transformed bacterial cultures at 42°C (108°F) will efficiently eliminate pJW168 and cease the production of cre recombinase.

The plnvRecA vector is designed to enable P1 transduction in any E.coli strain, making it highly useful in generating mutant strains for microbial genomic and proteomic studies, or for moving genes from one strain to another. This vector can be used in any E. coli strain, unlike other P1 transduction strategies, which can only be used with wild-type (RecA⁺) E. coli strains. The plnvRecA vector transiently converts RecA^- to RecA⁺. After transduction, the RecA^- phenotype can be completely restored by growing the cells at 42°C (108°F).

The pRANGER™-BTB vectors provide ultra-high stability cloning in a broad host-range expression vector. They incorporate the pBBR1 replicon to enable stable plasmid replication in Gram-negative bacteria. They also contain the pBAD promoter for inducible expression of cloned genes with a simple arabinose incubation. These features allow cloning and expression in many Gram-negative strains and avoid the structural and segregational instability that may occur with standard E. coli cloning vectors. pRANGER-BTB vectors are available in a choice of four antibiotic resistance cassettes.

The pSMART® cDNA-SS4 vector enables accurate and efficient production of single-stranded cDNAs for libraries, probes, and microarrays. The ligation-ready vector incorporates CloneSmart® technology for maximum insert stability with very low background, as well as the f1 origin for single-stranded DNA production, a T7 promoter for in vitro or in vivo expression, and convenient blunt+NotI cloning sites. The vector's cloning efficiency is greater than 99%, virtually eliminating the need for colony screening. It is provided dephosphorylated and precut at blunt-NotI sites in a 4X vector premix containing buffer and ATP.

The pTrueBlue® vector eliminates common problems in blue/white screening procedures by positioning the cloning sites within essential coding sequences that do not permit the formation of active beta-Gal fusion proteins. With the use of this vector, blue colonies can not be recombinants. The vector features 31 restriction sites for cloning, 8-base sites flanking the multiple clone site for clone mapping, ExoIII-protection sites for creation of unidirectional nested deletions, an f1 origin for ssDNA production, and a T7 promoter for making RNA probes. The vector has a high copy number (200-500 per cell) and can be used to clone inserts up to 3kb.

All vectors are provided as a 500µL E. coli glycerol stock containing the intact plasmid.

Specifications for Lucigen Ptrueblue Vector 42230-1:

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For a complete list of products offered by Lucigen please visit our Lucigen page. Don't forget to check out the extensive collection of Lucigen Polymerase chain reaction PCR cloning vectors or kits and a wide array of Polymerase chain reaction PCR cloning vectors or kits from other quality brands. You may reach us by phone, email or live chat! We have a 100% Satisfaction Guarantee to ensure that every laboratory product you receive is exactly what you are looking for! Contact us TODAY! Filling out our RFQ is very simple and we are more than happy to have the opportunity to work with your procurement department to get the best quote for any particular product you have in mind.