Pierce EZ-LIN S-NHS-SSBIOTIN KIT25MG 21445

Pierce EZ-LIN S-NHS-SSBIOTIN KIT25MG 21445 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Pierce EZ-LIN S-NHS-SSBIOTIN KIT25MG 21445 / PI21445 is part of a wide selection of Pierce Organic chemical mixtures. Combining excellent quality with an affordable price, the Pierce EZ-LIN S-NHS-SSBIOTIN KIT25MG 21445 / PI21445 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Organic chemical mixtures please do not hesitate to contact our customer service for further guidance.

The sulfo-NHS and NHS-PEO₄ kits make it easy to biotinylate antibodies or purified proteins. The reagents included link biotin to proteins via a stable, amide bond. The negative charge of the sulfonate group enhances the water solubility of the reagent, while preventing it from crossing cell membranes. These kits include Zeba* desalt spin columns for at least 85% protein recovery, more concentrated samples, and shorter processing time.

Solid-phase kits immobilize the selected antibody to a metal-chelated affinity support and perform the biotinylation in the solid phase. This protocol allows for potential enrichment, instead of dilution, of the IgG and limits over-biotinylation. It also makes it easy to remove spent or unreacted reagents and does not require any dialysis or desalting. Amine-directed biotinylation requires one hour, and sulfhydryl-directed biotinylation requires two hours. These kits are available in either a mini-spin format for 0.1-1.0mg IgG or a pre-packed column format for 1-10mg IgG.

Ordering Information: The sulfo-NHS kits contain enough reagents to run 10 labeling reactions. The solid-phase kits contain enough reagents to run eight labeling reactions.

Specifications for Pierce EZ-LIN S-NHS-SSBIOTIN KIT25MG 21445:

(Please note that the description and stock photo may not correspond to the specific model listed above)

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