Pierce Immobilized Glutathione Column 78250

Pierce Immobilized Glutathione Column 78250 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Pierce Immobilized Glutathione Column 78250 / PI78250 is part of a wide selection of Pierce Chemistry test kits or supplies. Combining excellent quality with an affordable price, the Pierce Immobilized Glutathione Column 78250 / PI78250 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Chemistry test kits or supplies please do not hesitate to contact our customer service for further guidance.

When proteins are expressed recombinantly, additional amino acids called fusion tags are often added to aid in purification and manipulation. These kits are available with six histidine residues (6xHis-tagged) or glutathione s-transferase (GST-tagged) fusion proteins. Kits are optimized to extract and purify the highest quality possible in the least amount of time. In addition, the protocols, do not require any manual or mechanical lysing steps. Instead kits include the appropriate cell lysis reagents.

Histidine-tagged recombinant proteins are easily processed because the tag binds to several metals, including nickel, cobalt, and copper. The agarose supports included in the histidine kits are derivatized with nickel chelating groups and iminodiacetic acid. This allows for a tighter bond with the histidine-tagged protein. GST-tagged recombinant proteins show more specific binding than those tagged with histidine, resulting in greater purity in the final samples. Because binding is so specific, the protocol requires the addition of reduced glutathione (included) to a physiologic buffer in order to release the GST fusion proteins from the immobilized glutathione substrates.

The B-PER* kits are designed for purification of bacterial cell lysates or baculovirus infected insect cells. The Y-PER* kit is designed to work with Saccharomyces cerevisiae, Schizosaccharomyces pombe, Bacillus subtilis, and E. coli.

Ordering Information: Kits come with all necessary reagents and substrates to run the protocols. Additional buffers, columns, and agarose are also available separately. For additional B-PER and Y-PER reagents, see the Poppers cell lysing reagents (PI78248 series).

Specifications for Pierce Immobilized Glutathione Column 78250:

(Please note that the description and stock photo may not correspond to the specific model listed above)

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