Pierce O-GLCNAC Western Blot 24565

Pierce O-GLCNAC Western Blot 24565 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Pierce O-GLCNAC Western Blot 24565 / PI24565 is part of a wide selection of Pierce Protein electrophoresis markers. Combining excellent quality with an affordable price, the Pierce O-GLCNAC Western Blot 24565 / PI24565 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Protein electrophoresis markers please do not hesitate to contact our customer service for further guidance.

O-Glycosidically-linked modifications of serine or threonine residues are found in proteins from nearly all eukaryotic cells. Although not precisely known, the function of the beta-O-linked N-acetylglucosamine (O-GlcNAc) modification may serve as an alternative to phosphorylation, as a modulator of protein interactions in multimeric complexes, or as a fortification against proteolytic attack. The kit contains a highly specific monoclonal mouse antibody that will only link to the beta modification. It does not show any cross-reactivity with the alpha version, the alpha/beta version, or any other N-linked oligosaccharides. The kit also includes SuperSignal* West dura extended duration substrate to allow for visualization of the modified proteins in less than one minute with a sensitivity to the low-picomole range.

Ordering Information: Kit includes 25mL M-PER* mammalian protein extraction reagent, two 50mL bottles of 10X dilution/blocking buffer, 17 packs BupH* PBS, three 10mL bottles Surfact-Amps* 20 (10% Tween*-20), 100µL Anti-O-GlcNAc monoclonal antibody in ascites, 75µg goat anti-mouse IgM(µ) HRP-conjugate, and 100mL SuperSignal West dura extended duration substrate. All reagents together are sufficient for 10 miniblots.

Specifications for Pierce O-GLCNAC Western Blot 24565:

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