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Western blot sensitivity is improved by reducing the amount of background in a system. High background is prevented by using blockers that prevent nonspecific binding and optimize the signal-to-noise ratio. StartingBlock* buffers are biotin- and serum-free. They are compatible with many detection systems and feature short blocking times. These buffers also allow stripping and reprobing with reblocking to save time and money. SuperBlock* buffers are biotin- and serum-free and feature a stabilizing formula that allows blocked plates to be stored dry for up to 12 months. SEA BLOCK buffer contains no mammalian proteins to reduce the risk of nonspecific interaction. It is made instead from steelhead salmon serum and functions as a universal blocker. It can be diluted up to 1:10 in either PBS or TBS. Blocker* buffers are preformulated solutions that are ready-to-use. BLOTTO buffer is made with non-fat dry milk. BSA buffers are concentrated 10% solutions of bovine serum albumin for all blocking applications. Casein buffer is 1% (w/v) Hammarsten grade casein designed for blocking nonspecific sites. BupH* buffers are dry buffer packs that can be reconstituted to create unique blocking buffers or used on their own as high-purity wash buffers for Western blotting. Restore* Western blot stripping buffer saves time and samples by allowing reprobing of the original membrane using the same target sample. Gentle, odor-free, proprietary formulation provides efficient removal and does not damage target protein after stripping and reprobing.
Description: | PBS |
Application: | ELISA, Western Blot, Dot Blot, Immunohistochemistry |
Size: | 1 L |
Unit: | Each |