Quanta Biosciences Qscript 1-STP Fast Mgb 100R 95082-100

Quanta Biosciences Qscript 1-STP Fast Mgb 100R 95082-100 is one of the many quality laboratory and scientific products we have to offer at very competitive pricing. Quanta Biosciences Qscript 1-STP Fast Mgb 100R 95082-100 / 95039-946 is part of a wide selection of Quanta Biosciences Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction. Combining excellent quality with an affordable price, the Quanta Biosciences Qscript 1-STP Fast Mgb 100R 95082-100 / 95039-946 can meet your scientific research needs while still offering a good value for a budget conscious end user. If you have any questions about any of our Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction please do not hesitate to contact our customer service for further guidance.

Kits offer a convenient and highly sensitive solution for real-time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridization probe detection chemistries such as TaqMan* 5'-hydrolysis probes or molecular beacons on PCR instrument platforms.

For Minor Groove Binder (MGB) modified probes, qScript* One-Step fast MGB qRT-PCR kits are recommended. These kits offer a convenient and highly sensitive solution for qRT-PCR of RNA templates using TaqMan-MGB 5'-hydrolysis probe detection exclusively on qRT-PCR instrument platforms.

The system allows cDNA synthesis and PCR amplification to be carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets, as wells as high throughput gene-expression studies. It has also been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.

One-step fast master mixes are provided as a 4x concentrated solution to allow addition of higher amounts of RNA template and improved detection sensitivity with low concentration samples. The unique formulation maximizes the activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts. This enables unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes in duplexed qRT-PCR applications.

Highly-specific amplification is crucial to successful qRT-PCR, as non-specific product(s) can compete for amplification of the target sequence and impair PCR efficiency. A key component of these kits is AccuStart* Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive during reaction assembly and the 50°C reverse transcription step. A brief 30-second heat activation step at 95°C irreversibly denatures the antibodies, releasing fully active, unmodified Taq DNA polymerase. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Therefore, these kits afford greater reagent economy and laboratory throughput on conventional or rapid ramp rate qPCR systems.

Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX* reference dye. Platforms not requiring passive reference dye include Roche LightCycler* 480, Bio-Rad* iQ*, MyiQ*, iQ5, Opticon*, Chromo4*, Corbett Rotor-Gene*, Eppendorf* Mastercycler*, and Cepheid* SmartCycler*. Kits with Low ROX reference dye are for use with platforms including AB 7500 and Stratagene* Mx*. Kits with ROX reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne*.

Specifications for Quanta Biosciences Qscript 1-STP Fast Mgb 100R 95082-100:

(Please note that the description and stock photo may not correspond to the specific model listed above)

For a complete list of products offered by Quanta Biosciences please visit our Quanta Biosciences page. Don't forget to check out the extensive collection of Quanta Biosciences Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction and a wide array of Kits for messenger ribonucleic acid mRNA quantitation by polymerase chain reaction from other quality brands. You may reach us by phone, email or live chat! We have a 100% Satisfaction Guarantee to ensure that every laboratory product you receive is exactly what you are looking for! Contact us TODAY! Filling out our RFQ is very simple and we are more than happy to have the opportunity to work with your procurement department to get the best quote for any particular product you have in mind.